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BackgroundRetinal degeneration is a major cause of irreversible blindness. Stimulation with controlled low-level electrical fields, such as transcorneal electrical stimulation (TES), has recently been postulated as a therapeutic strategy. With promising results, there is a need for detailed molecular characterization of the therapeutic effects of TES. MethodsControlled, non-invasive TES was delivered using a custom contact lens electrode to the retinas of Royal College of Surgeons (RCS) rats, a model of retinal degeneration. DNA methylation in the retina, brain and cell-free DNA in plasma was assessed by reduced representation bisulfite sequencing (RRBS) and gene expression by RNA sequencing. ResultsTES induced DNA methylation and gene expression changes implicated in neuroprotection in the retina of RCS rats. We devised an epigenomic-based retinal health score, derived from DNA methylation changes observed with disease progression in RCS rats, and showed that TES improved the epigenomic health of the retina. TES also induced DNA methylation changes in the superior colliculus: the brain which is involved in integrating visual signaling. Lastly, we demonstrated that TES-induced retinal DNA methylation changes were detectable in cell-free DNA derived from plasma. ConclusionTES induced DNA methylation changes with therapeutic effects, which can be measured in circulation. Based on these changes, we were able to devise a liquid biopsy biomarker for retinal health. These findings shed light on the therapeutic potential and molecular underpinnings of TES, and provide a foundation for the further development of TES to improve the retinal health of patients with degenerative eye diseases. 

ABSTRACT J191213.72 − 441045.1 is a binary system composed of a white dwarf and an M-dwarf in a 4.03-h orbit. It shows emission in radio, optical, and X-ray, all modulated at the white dwarf spin period of 5.3 min, as well as various orbital sideband frequencies. Like in the prototype of the class of radio-pulsing white dwarfs, AR Scorpii, the observed pulsed emission seems to be driven by the binary interaction. In this work, we present an analysis of far-ultraviolet spectra obtained with the Cosmic Origins Spectrograph at the Hubble Space Telescope, in which we directly detect the white dwarf in J191213.72 − 441045.1. We find that the white dwarf has a temperature of Teff = 11485 ± 90 K and mass of 0.59 ± 0.05 M⊙. We place a tentative upper limit on the magnetic field of ≈50 MG. If the white dwarf is in thermal equilibrium, its physical parameters would imply that crystallization has not started in the core of the white dwarf. Alternatively, the effective temperature could have been affected by compressional heating, indicating a past phase of accretion. The relatively low upper limit to the magnetic field and potential lack of crystallization that could generate a strong field pose challenges to pulsar-like models for the system and give preference to propeller models with a low magnetic field. We also develop a geometric model of the binary interaction which explains many salient features of the system. 

ABSTRACT Blue Large-Amplitude Pulsators (BLAPs) are a relatively new class of blue variable stars showing periodic variations in their light curves with periods shorter than a few tens of minutes and amplitudes of more than 10 per cent. We report nine blue variable stars identified in the OmegaWhite survey conducted using ESO’s VST, which shows a periodic modulation in the range 7–37 min and an amplitude in the range 0.11–0.28 mag. We have obtained a series of followup photometric and spectroscopic observations made primarily using SALT and telescopes at SAAO. We find four stars which we identify as BLAPs, one of which was previously known. One star, OW  J0820–3301, appears to be a member of the V361 Hya class of pulsating stars and is spatially close to an extended nebula. One further star, OW J1819–2729, has characteristics similar to the sdAV pulsators. In contrast, OW J0815–3421 is a binary star containing an sdB and a white dwarf with an orbital period of 73.7 min, making it only one of six white dwarf-sdB binaries with an orbital period shorter than 80 min. Finally, high cadence photometry of four of the candidate BLAPs show features that we compare with notch-like features seen in the much longer period Cepheid pulsators. 

Abstract The Vera C. Rubin Legacy Survey of Space and Time (LSST) holds the potential to revolutionize time domain astrophysics, reaching completely unexplored areas of the Universe and mapping variability time scales from minutes to a decade. To prepare to maximize the potential of the Rubin LSST data for the exploration of the transient and variable Universe, one of the four pillars of Rubin LSST science, the Transient and Variable Stars Science Collaboration, one of the eight Rubin LSST Science Collaborations, has identified research areas of interest and requirements, and paths to enable them. While our roadmap is ever-evolving, this document represents a snapshot of our plans and preparatory work in the final years and months leading up to the survey’s first light. 

Abstract We present very early photometric and spectroscopic observations of the Type Ia supernova (SN Ia) 2023bee, starting about 8 hr after the explosion, which reveal a strong excess in the optical and nearest UV (UandUVW1) bands during the first several days of explosion. This data set allows us to probe the nature of the binary companion of the exploding white dwarf and the conditions leading to its ignition. We find a good match to the Kasen model in which a main-sequence companion star stings the ejecta with a shock as they buzz past. Models of double detonations, shells of radioactive nickel near the surface, interaction with circumstellar material, and pulsational delayed detonations do not provide good matches to our light curves. We also observe signatures of unburned material, in the form of carbon absorption, in our earliest spectra. Our radio nondetections place a limit on the mass-loss rate from the putative companion that rules out a red giant but allows a main-sequence star. We discuss our results in the context of other similar SNe Ia in the literature. 

Aims. With the aim of interpreting anomalous lensing events with no suggested models, we conducted a project of reinvestigating microlensing data collected in and before the 2019 season. In this work, we report a multi-planet system, OGLE-2019-BLG-0468L, that was found as a result of this project. Methods. The light curve of the lensing event OGLE-2019-BLG-0468, which consists of three distinctive anomaly features, could not be explained by the usual binary-lens or binary-source interpretations. We find a solution that explains all anomaly features with a triple-lens interpretation, in which the lens is composed of two planets and their host, making the lens the fourth multi-planet system securely found by microlensing. Results. The two planets have masses of ~3.4  M J and ~10.2  M J , and they are orbiting around a G-type star with a mass of ~0.9  M ⊙ and a distance of ~4.4 kpc. The host of the planets is most likely responsible for the light of the baseline object, although the possibility of the host being a companion to the baseline object cannot be ruled out. 

The anamniote lateral line system, comprising mechanosensory neuromasts and electrosensory ampullary organs, is a useful model for investigating the developmental and evolutionary diversification of different organs and cell types. Zebrafish neuromast development is increasingly well understood, but neither zebrafish nor Xenopus is electroreceptive and our molecular understanding of ampullary organ development is rudimentary. We have used RNA-seq to generate a lateral line-enriched gene-set from late-larval paddlefish (Polyodon spathula). Validation of a subset reveals expression in developing ampullary organs of transcription factor genes critical for hair cell development, and genes essential for glutamate release at hair cell ribbon synapses, suggesting close developmental, physiological and evolutionary links between non-teleost electroreceptors and hair cells. We identify an ampullary organ-specific proneural transcription factor, and candidates for the voltage-sensing L-type Cav channel and rectifying Kv channel predicted from skate (cartilaginous fish) ampullary organ electrophysiology. Overall, our results illuminate ampullary organ development, physiology and evolution.